PCR with very low DNA concentrations

Lord Antarchon MDC128 at psuvm.psu.edu
Fri Jun 16 14:10:43 EST 1995


Is there a established strategy one follows when trying to get PCR
product from low concentration DNA, i.e., you increase DNA concentration
first, then primers, etc. I am trying to amplify a low-expression gene
from a lambda gt11-based cDNA library.  If anyone has a clue, please
email mdc128 at psuvm.psu.edu, because I'm starting to get a bit vexed with
my reactions.



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