Single-stranded DNA prep reference?
yoshimum at essex.hsc.colorado.edu
Fri Jun 16 12:14:45 EST 1995
On Thu, 15 Jun 1995, Josh Goldstein wrote:
> Jupin and Gronenborn, 1995. Abundant, easy and reproducible production
> of ssDNA from phagemids using helper phage-infected competent cells.
> Nucleic Acids Research, 23:3, pp.535-536
> You know, I tried this myself and it didn't work. They used the
> hexamminecobalt chloride method of preparing competent cells, while I used
> CaCl, so maybe that was the problem. Let me know if it works for you.
> Josh Goldstein
> josh at panda.uchc.edu
I read this paper and thought that it was a neat idea. However, the
photo they showed in Fig. 1 made me wonder. In my hand and references I
know, circular ssDNA migrates faster than corresponding super-coiled
dsDNA. The bands in the Fig look too big. Are they something else?
Please correct me, if I am wrong. Masami.
More information about the Methods