rafael at corona.med.utah.edu
Thu Jun 15 19:26:29 EST 1995
On 15 Jun 1995, Garth I. Patterson wrote:
> I am interested in performing a mutagenesis with something that will give a
> different spectrum of changes from EMS, which gives primarily G-C to A-T
> transitions, at least in C. elegans (my favorite beast). I want single
> base pair changes, rather than deletions or frame shifts. As usual, I
> will post a
> summary of comments/suggestions.
> Garth Patterson
Most of the mutangens give GC to AT transitions, but the base analog
2-aminopurine should give GC to AT and AT to GC as well.
Benzo[a]pyrene diolepoxide is a very dangerous one and difficult to
obtain, but can make GC to TA transversions. 4-nitro-quinoline-1-oxide
mainly gives GC to AT, but some GC to TA too.
Those mutations are in E. coli, I don't know how they will work in your
I hope this helps.
Rafael Maldonado | "y es que...
room 6160 Eccles Institute of Human Genetics | habemos gente pa to'"
Department of Human Genetics |
University of Utah |
Salt Lake City, Utah 84112. USA. | Joselito "El Gallo"
Rafael at genetics.med.utah.edu |
Rafael at corona.med.utah.edu |
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