Southern blots

Martin Leach leach at bu.edu
Fri Jun 16 08:42:43 EST 1995


I agree with the previous post...

However, I use Denhardt's Hyb solution....and stick to hyb's and 
prehyb's at 65C....again, I agree dont be too stringent in your 
washes...

check the blots after each wash...

start at RT in 0.5xssc/0.1%sds then move to
40C in 0.2xssc/0.1%sds

and then to 

55C in 0.1xssc/0.1%sds

check the blots at each step....if the counts get too low then dont wash 
any more....

ciao

Martin
....             Martin Leach                Email:leach at bu.edu 
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