Problem with phenol
rafael at corona.med.utah.edu
Fri Jun 16 13:48:33 EST 1995
On Fri, 16 Jun 1995, Ramachandran wrote:
> Is there anyone who can tell me about the kind of phenol that should be
> used for isolating genomic DNA from mouse tails. Is there some kind of
> time tested special method to buffer the phenol?
> And I have been trying to bring down the pH of phenol (to be used in DNA
> isolation) with several washes of 0.1 M Tris-Cl, without any success. I
> have done almost 7 washes with 0.1 M Tris-Cl. I am following the
> protocol given in the Maniatis Lab mannual. If someone can suggest a
> solution it will be great!
If you read the maniatis protocol, it says you should wash the phenol
with 1 M Tris-HCl first. Try several (say, 3) with 1 M and other 3 washes
with 0.1 M. The pH must be OK then (between 7 and 8).
Rafael Maldonado | "y es que...
room 6160 Eccles Institute of Human Genetics | habemos gente pa to'"
Department of Human Genetics |
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