Glycerol stocks at -20?
colin_rasmussen at darwin.biochem.ualberta.ca
Sat Jun 17 15:08:47 EST 1995
vwarwar at unlinfo.unl.edu (vitor warwar) wrote:
> Hi all:
> Does anybody knows of bacteria glycerol stocks could be
> stored at -20 instead of -70?
Yes they can. We often do this for short-term (months) storage of things
where we are sorting out which ones we will finally keep. For long-term
storage (years) -70°C is better. We have found that viability at -20°C
doesn't seem as good and I would hate to come back to something after a
few years to find the bugs had died. If this does happen, you can often
recover the plasmid by taking the seemingly dead glycerol stock, do a
mini-prep to isolate plasmid and retransform into competent bacteria. We
have recovered more than one clone in this way.
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