Nicked mitochondiral DNA: how to avoid?
Mike-Anson at NIH.GOV
Sat Jun 17 12:58:12 EST 1995
We've been trying to isolate mitochondrial DNA
from rat liver. We've tried several protocols,
but in general, we homogenize the tissue,
isolate the mitochondria by differential
centrifugation, lyse with an SDS-containing
buffer, and purify the DNA in one of the usual
ways. The details don't seem to change the
final result: nearly 100% open-circle, no
Has anyone encountered this problem, and found
Suggestions would be greatly appreciated!
Thanks in advance.
Mike-Anson at nih.gov
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