ethidium staining of RNA gels
Mike-Anson at NIH.GOV
Sat Jun 17 12:48:46 EST 1995
In article <kgweinst-1206951357230001 at 18.104.22.168>, kgweinst at tigr.org says...
>We have had several discussions in our laboratory about the transfer of
>RNA (for Northerns) from gels stained with ethidium bromide. Although
>several of the lab books (e.g. Maniatis/Sambrook) recommend against it, no
>RNA from agarose gels. Is there another stain which would allow staining
>of the RNA with no effect on transfer.
>Keith G. Weinstock
>The Institute for Genomic Research
>kgweinst at tigr.org
Is it essential to stain *in* the gel? Maniatis/Sambrook also gives a method for staining the membrane *after*
transfer, with methylene blue. The stained membrane can be photographed or photocopied, then destained. I
haven't done a Northern in a long time, but used to use that technique with good results.
Mike-Anson at nih.gov
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