DNA-biotinylated protein interaction

at at
Mon Jun 19 20:19:35 EST 1995


Is there anybody knows the method to determine DNA-protein interaction
using biotinylation of protein? I am going to see whether my protein
interacts directly with  the promoter of a specific gene. So, I am going
to do in vitro translation of my protein using biotin labelled tRNA-lysine
and mix with radioactive labelled DNA probe to see the interaction of
DNA-protein. Would you please send me the protocol for this study? Or if
you know the literature I can refer, please let me know.
My e-mail address is 
ilha_lee at qm.salk.edu
I will really appreciate for your help.



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