Help! Quantification of total RNA, equal loading

Aida Cancel axc19 at
Mon Jun 19 21:35:20 EST 1995

An easier way to quantitate total RNA is to do a densitometer scanning of the gel, 
or a negative of the gel.  After scanning you can quantitate the peaks and compare 
the amounts of 16 s etc. units that you get.  I used this technique in a paper that 
was published a year ago (meaning that it can be used for publication).  If you want 
to check how precise it is, just do a hybridization with a known constant probe.


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