Recovery of DNA from nylon membrane

Damak, Sami damak at whio.lincoln.ac.nz
Mon Jun 19 06:04:24 EST 1995


I did a plaque lift from plasmid-transformed E.coli, hybridized them and got 
some positive colonies.  The problem is all my colonies died and I didn't do 
replica.  My question is:  is there anyway of recovering the plasmid DNA 
from the membrane (Hybond N+) (which I have already dried).
Any suggestion will be appreciated.
Magda



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