Autosequence machinery

Bengt Oxelman bengt.oxelman at
Mon Jun 19 08:48:58 EST 1995

Having got a grant for buying automated sequencing equipment, we would
appreciate any comments on the pro's and cons of ABI 377 Prism,
Pharmacia's ALF and LI-COR. We will mainly use the stuff for sequencing
PCR-generated fragments (ds). Just reading the manufacturers own
description makes one suggest that the ddNTP-labelling strategy of ABI is
superior, but does it work and haw many bases can one routinely read from
one reaction?

Thank You,


Bengt Oxelman
Dept. of Systematic Botany
Carl Skottsbergs Gata 22
S-413 19 Goeteborg
bengt.oxelman at 

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