losing my plasmid

Martin Leach leach at bu.edu
Mon Jun 19 07:35:18 EST 1995

Try switching to DH5-alpha.....I had lots of problems with xl1-blue 
cells when I was cloning DNA (non-promoter) that contained repeats....I 
know repetitive DNA is notorious for giving problems when cloning...but 
the switch to DH5-alpha helped....


....             Martin Leach                Email:leach at bu.edu 
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