Xenopus oocyte expression

Lely Chow lelyc at u.washington.edu
Tue Jun 20 14:41:07 EST 1995


Hi All!

Does anyone know of reasons why expression would not occur after either 
injection of cDNA into the oocyte nucleus, or injection of mRNA into the 
cytoplasm?  In control injections using other DNA/RNA, we do get 
expression, so the problem must be something intrinsic to the RNA or 
protein itself.  Maybe something to do with mRNA stability??? 



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