quantitation of western blots
william at neuro.usc.edu
Tue Jun 20 20:54:53 EST 1995
Does anyone out there know if the signal observed on a western blot is
linear with the amount of protein loaded on a gel? Does this linearity
depend on the detection method used? ie. radioactive vs chemical detection.
I am using secondary antibody conjugated to alkaline phosphatase. I
visulize the protein by adding NBT substrate. Thanks for any information.
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