quantitation of western blots

William Sun william at neuro.usc.edu
Tue Jun 20 20:54:53 EST 1995


Hello all,

Does anyone out there know if the signal observed on a western blot is
linear with the amount of protein loaded on a gel?  Does this linearity
depend on the detection method used?  ie. radioactive vs chemical detection.
I am using secondary antibody conjugated to alkaline phosphatase.  I 
visulize the protein by adding NBT substrate.  Thanks for any information.

-William




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