Biotinylation of dsDNA
jmsst88+ at pitt.edu
Tue Jun 20 15:38:33 EST 1995
In article <3rmmi0$9n at hippo.shef.ac.uk>, M.C.Behrendt at shef.ac.uk
> Could anyone tell me how to biotinylate dsDNA (other than mixing the
> dsDNA with photobiotin and puting it under a 400W UV lamp)
> that is both easy and cheap. I need to have approximately every 80th
> base biotinylated. The DNA is from any old plasmid that happens to be
> lying about.
Unfortunately, I have only another question in response to your post. I
guess that you have had some experience(and success?) with photobiotin.
Besides looking for a red pellet following EtOH precipitation, are there
any other means of detecting successful biotinylation? I am presently
attempting to dot blot my nucleic acid onto nitrocellulose and "probe"
with conjugated avidin-HRP for detection by ECL. Any other suggestions?
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