GST Recombinant Proteins -- HELP!

Rick Davis rick at unity.ncsu.edu
Wed Jun 21 10:46:17 EST 1995


I have been trying to purify a recombinant peptide using the Pharmacia GST
fusion protein expression kit.  Trying to recover pure recombinant peptide
by cleaving the GST with Factor Xa and immobilize it on GT-agarose is
incomplete no matter how many passes over the column.  Is there a way to
completely remove cleaved GST to achieve pure recombinant peptide (free of
GST)?  Any suggestions would be greatly appreciated.  Rick

-- 
rick at unity.ncsu.edu



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