Riboprobes

Tracy Aquilla aquilla at salus.med.uvm.edu
Wed Jun 21 06:43:43 EST 1995


In Article <1995Jun19.100817.54281 at cc.usu.edu>, sl6dp at cc.usu.edu wrote:
>Does anybody know if there is a minimum length for a good riboprobe?
>I am thinking of making 300bp max. riboprobes, is there any drawbaks for 
>this, any special meassures to consider???

I routinely make riboprobes over 500 bp with no problems. Longer probes will
give greater sensitivity in an RNAse protection assay, but can give some
problems if the RNA being assayed is somewhat degraded.
    tracy



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