Help - Lysogenizing Lambda into E. coli

MID schryver at acs.ucalgary.ca
Tue Jun 20 23:19:16 EST 1995


I am trying to create lambda lysogens in tonB mutant E. coli.

I thought that it would be a piece of cake to find plenty of different
procedures to do this, but I am having a hell of a time.

I am trying to develop a protocol for my system but I would really like to
find some that have been done already, this may help me to avoid the
pitfalls of those that have gone before.

Thanks for any help you can give me

Kurt "sims at acs.ucalgary.ca"



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