Cross-linking RNA to Hybond-N membranes

Karim Sayani ksayani at ualberta.ca
Fri Jun 23 01:03:36 EST 1995


hello everyone,

I'm looking for info. on UV X-linking of RNA onto nylon membranes after
the RNA has been transferred from a gel (ie Northern blotting).  I need
to know how long and what energy level is required for effectively 
binding the RNA to the membrane.  As well,  how does this compare with 
the conventional baking at 80C.

Any ideas or experience?



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