Cross-linking RNA to Hybond-N membranes
ksayani at ualberta.ca
Fri Jun 23 01:03:36 EST 1995
I'm looking for info. on UV X-linking of RNA onto nylon membranes after
the RNA has been transferred from a gel (ie Northern blotting). I need
to know how long and what energy level is required for effectively
binding the RNA to the membrane. As well, how does this compare with
the conventional baking at 80C.
Any ideas or experience?
More information about the Methods