insoluble GST-fusion protein
plxigpg at pln1.nottingham.ac.uk
Thu Jun 22 07:13:44 EST 1995
I am having a bit of a problem with a GST-fusion protein which is
insoluble. We have tried sarkosyl treatment to solubilize and found
that it does solubilize in 0.25%-0.5% but we cannot get it to bind to
the resin!!! We have tried adding triton X100 (1%-5%) but still no
fusion - although the GST binds ok!!
Any suggestions would be most appreciated!
Thanks in advance,
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