insoluble GST-fusion protein

Ian Goodfellow plxigpg at pln1.nottingham.ac.uk
Thu Jun 22 07:13:44 EST 1995


Dear all,

I am having a bit of a problem with a GST-fusion protein which is
insoluble.  We have tried sarkosyl treatment to solubilize and found 
that it does solubilize in 0.25%-0.5% but we cannot get it to bind to 
the resin!!!  We have tried adding triton X100 (1%-5%) but still no 
fusion - although the GST  binds ok!!

Any suggestions would be most appreciated!

Thanks in advance,

Ian





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