Jack M. Bernstein, M.D.
bernstein at wsu-id.dayton.oh.us
Thu Jun 22 13:59:37 EST 1995
In article <3sb3s3$d0f at metro.ucc.su.OZ.AU> pwhmg02 at angis.su.oz.au (Prince of Wales Endocrinology Unit) writes:
>From: pwhmg02 at angis.su.oz.au (Prince of Wales Endocrinology Unit)
>Subject: Triton X-100
>Date: 22 Jun 1995 06:50:43 GMT
>Has anybody a successful method for removing Triton X-100
>from samples which will be loaded onto SDS PAGE? We have
>found that the Triton distorts the lanes.
>Heather (pwhmg02 at angis.su.oz.au)
This has been noted for many years. Possible solutions include diluting your
sample or using something other than TX100 (eg: octylglucoside). One
suggestion is to make sure that all your lanes have the same concentration of
TX100 in them. Then the artifact will be more uniform.
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