Inclusion bodies (aaaaahhhhhhh!!!!)

jl at dna.bio.warwick.ac.uk jl at dna.bio.warwick.ac.uk
Sun Jun 25 10:07:57 EST 1995


I am trying to express a monooxygenase in different bacterial strains
including E. coli.  Although expression from the T7 promoter
is seen, the protein seems to aggregate as inclusion bodies and is
inactive.

Many methods such as fusion proteins and solubilisation of these
inclusion bodies have been reported and are to be attempted.

Can I hear your ideas and views on getting over this problem? 

Thanks for your help!

John Lloyd
Biological Sciences
University of Warwick
UK

JL at dna.bio.warwick.ac.uk 




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