PCR cloning

Michael Clark michael-clark at uiowa.edu
Fri Jun 23 18:48:38 EST 1995

Junhyong said:

>I heard somewhere that there are now other commercial T-tailed vectors
>available for PCR product cloning than TA-cloning kit from Invitrogen.
>Has anybody tried others? Is it cheaper?

We've been using Promega's T-vector system (pGEM-T) for quite a while
now with *excellent* results, much better than the Invitrogen system. 
It if definetly worth the $$$.  And no, I'm not a company rep, we've
just had really good luck with it.

Michael Clark
University of Iowa
Dept. of Physiology

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