Library problem
Jiasui Zhan
j0z1480 at sigma.tamu.edu
Sat Jun 24 13:11:55 EST 1995
I have a problem with construction of a genomic DNA library. I
constructed a genomic library in pUC18. I plated my library out on LB
plates with amp/X-gal/IPTG. I got about 200 white colonies, this looked
good enough for my purposes. That same day I picked 40 of the white
colonies onto another LB plate with amp/X-gal/IPTG and I also picked one
blue colony as a control. To my surprise the next day non of the white
colonies grew but the blue colony grew fine. I have no idea what happened
and what to do next. Could someone Please Please help me!!!! I would
gratefully appreciate any help. Thank you very much.
Rodney Earl Pettway
Jiasui Zhan
Dep. of Plant Pathology & Microbiology
Texas A&M University
College Station, TX77843
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