Stripping a blot

Graham Dellaire popa0206 at PO-Box.McGill.CA
Sat Jun 24 12:04:17 EST 1995

>   KRIS B <KXB18 at> writes:
>  I want to strip a western to reuse it. I tried a solution that consisted of 62.
>  5mM Tris, 2% SDS and 100 mM mercaptoethanol. I incubated the blot in this solut
>  ion for 30 min at 70 C. It didn't work. Help! I'd appreciate any input..Thanks
>  Kris
You can up the sds % to 5 and you can increase the temperaturein increments of 5 degrees....
you want more stringency right.... 

Graham Dellaire			  

More information about the Methods mailing list