Stripping a blot

[Graham Dellaire]

>   KRIS B <KXB18 at psuvm.psu.edu> writes:
>  I want to strip a western to reuse it. I tried a solution that consisted of 62.
>  5mM Tris, 2% SDS and 100 mM mercaptoethanol. I incubated the blot in this solut
>  ion for 30 min at 70 C. It didn't work. Help! I'd appreciate any input..Thanks
>  Kris
>  
>>>>
You can up the sds % to 5 and you can increase the temperaturein increments of 5 degrees....
you want more stringency right.... 


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Graham Dellaire