efficient blunt ligation?
mike.holloway at stjude.org
Mon Jun 26 13:33:31 EST 1995
In article <3sdpek$n42 at worak.kaist.ac.kr>, hbkim at biochem.kaist.ac.kr (Hanbok Kim) says:
>Is there any effective method for blunt end ligation
>by temperature regulation
Try the Boehringer Mannheim Rapid DNA ligation kit. Their buffer comes
with some viscous secret ingredient (PEG + something else?) and a fairly
high concentration ligase (5u/ul). Instructions say 5 minutes at room temp,
sticky or blunt end. So far, its working as advertised. They also insist
that you use high efficiency hexamminecobalt chloride method competent
cells, which would have an affect on the success of any ligation project.
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