DDRT-PCR Northerns

McMaster Lab genes at unixg.ubc.ca
Mon Jun 26 10:11:28 EST 1995

Because DDRT-PCR is very sensitive, you may be isolating single copy 
mRNA.So,unless you have a screaming hot probe and enough RNA on the 
Northern to swim in, you will never see a band on the autorad.
This of course is no help to you. We tried reverse Northerns but never 
had much success. Try S1 nuclease protection kit by Ambion.
Good Luck.
Craig Kreklywich
Med. Genetics 

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