genes at unixg.ubc.ca
Mon Jun 26 10:11:28 EST 1995
Because DDRT-PCR is very sensitive, you may be isolating single copy
mRNA.So,unless you have a screaming hot probe and enough RNA on the
Northern to swim in, you will never see a band on the autorad.
This of course is no help to you. We tried reverse Northerns but never
had much success. Try S1 nuclease protection kit by Ambion.
More information about the Methods