Inclusion bodies (aaaaahhhhhhh!!!!)

[Henning Saggau]

In article <3sju4d$bbv at violet.csv.warwick.ac.uk> jl at dna.bio.warwick.ac.uk writes:
>From: jl at dna.bio.warwick.ac.uk
>Subject: Inclusion bodies (aaaaahhhhhhh!!!!)
>Date: 25 Jun 1995 15:07:57 GMT

>I am trying to express a monooxygenase in different bacterial strains
>including E. coli.  Although expression from the T7 promoter
>is seen, the protein seems to aggregate as inclusion bodies and is
>inactive.

>Many methods such as fusion proteins and solubilisation of these
>inclusion bodies have been reported and are to be attempted.

>Can I hear your ideas and views on getting over this problem? 

Have U tried to add MgCl2 to the media? This should slow down the growth of 
the E.coli. It might have  a positive effect but not in every case.
Try it.

Henning
saggau at ps1515.chemie.uni-marburg.de