Two-Hybrid-System: How get started

Stephan Witte stephan.witte at
Tue Jun 27 04:59:58 EST 1995

I«m studying some protein protein interactions. I«ve read a lot about about
the yeast-two-hybrid system.
And now i«d like to start.
Are there some good manuals/references? Where to buy the vectors, strains
(which?) Are there good kits?
What things should be avoided? Hints and tips?

Thanks a lot for any help


Stephan Witte
Inst. of Immunology
University of Constance
stephan.witte at

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