Need supplier for vacuum apparatus
Jeffrey K. Harrison
jharrison at qm.server.ufl.edu
Tue Jun 27 12:16:23 EST 1995
In article <3smnn8$n9i at rover.ucs.ualberta.ca>, Colin Rasmussen
<colin_rasmussen at darwin.biochem.ualberta.ca> wrote:
> sullivan at gwis2.circ.gwu.edu (Steven Sullivan) wrote:
> >You know when you are checking incorporation of hot nukes into a cDNA,
> >and you do a TCA precipitation and wash on little round Whatman fiber
> >discs? I need to find a company that sells the vacuum attachment that
> >holds the Whatman discs while you wash them -- I recall it being a
> >polished metal, cylinder-shaped manifold type thing in two parts, that
> >sandwich the disc; you fit it into a rubber stopper and use a ported
> >Erlenmeyer as a catch basin for the flow-through.
> We use a set-up that is made by Millipore. Ours consists of a lower
> glass piece that fits into a stopper in the side-arm Erlenmeyer, a metal
> ring/screen that the filter goes on, and an upper glass chamber that you
> can pipette in the sample.
Your "polished metal, cylinder-shaped maifold type thing in two parts" can
be purchased from Hoeffer. They come in two sizes, one for 24 mm and
another for 25 mm filters (cat #s FH 124 and FH 125).
Jeffrey K. Harrison, Ph.D.
University of Florida
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