Two-Hybrid-System: How get started
myagi at u.washington.edu
Tue Jun 27 12:59:30 EST 1995
Clontech makes a pretty good kit; we've used it so far with good
results. Stratagene alson now has a modified version of lambda ZAP
containing an activation domain plasmid; we're in the process of using
this to generate a cDNA library. The usual disclaimers apply.
On 27 Jun 1995, Stephan Witte wrote:
> I«m studying some protein protein interactions. I«ve read a lot about about
> the yeast-two-hybrid system.
> And now i«d like to start.
> Are there some good manuals/references? Where to buy the vectors, strains
> (which?) Are there good kits?
> What things should be avoided? Hints and tips?
> Thanks a lot for any help
> Stephan Witte
> Inst. of Immunology
> University of Constance
> stephan.witte at uni-konstanz.de
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