DNA purification from agarose gels
Holger at gem.univie.ac.at
Tue Jun 27 11:49:30 EST 1995
In article <3sp9rk$1tp at lyra.csx.cam.ac.uk>, rw200 at cus.cam.ac.uk (R.
> Dear all
> I would like to canvas opinion on the best kit or perhaps method
> when purifying DNA fragments from agarose gels, at the moment we
> 'EluQuik' kit from S&S the yield is good and so is the purity but its
> alittle fiddly especially if you perform alot of isolations at
> . I want to use the purified fragments in ligation reactions.
> Thanks to all Robert
> Dept Pharmacology
> Tennis Court Rd
> University of Cambridge
> Email rw200 at cus.cam.ac.uk
The easiest and cheapest method I think is "Gene Clean". You just need 6M
NaI and glasmilk to get pure and good DNA for cloning experiments.
E-mail: holger at gem.univie.ac.at
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