DNA purification from agarose gels

Holger Scholz Holger at gem.univie.ac.at
Tue Jun 27 11:49:30 EST 1995


In article <3sp9rk$1tp at lyra.csx.cam.ac.uk>, rw200 at cus.cam.ac.uk (R.
Woodward) wrote:

> Dear all
>         I would like to canvas opinion on the best kit or perhaps method
to use
>         when purifying DNA fragments from agarose gels, at the moment we
use the
>         'EluQuik' kit from S&S the yield is good and so is the purity but its
>         alittle fiddly especially if you perform alot of isolations at
one time
>         . I want to use the purified fragments in ligation reactions.
>         Thanks to all Robert
> 
>         R.Woodward
>         Dept Pharmacology
>         Tennis Court Rd
>         University of Cambridge
>         Email rw200 at cus.cam.ac.uk


The easiest and cheapest method I think is "Gene Clean". You just need 6M
NaI and glasmilk to get pure and good DNA for cloning experiments.
  
     Holger


H.Scholz
E-mail: holger at gem.univie.ac.at



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