DNA purification from agarose gels
tedm at darkwing.uoregon.edu
Wed Jun 28 01:23:35 EST 1995
In article <3sp9rk$1tp at lyra.csx.cam.ac.uk>, rw200 at cus.cam.ac.uk (R.
> Dear all
> I would like to canvas opinion on the best kit or perhaps method
> when purifying DNA fragments from agarose gels,
Quick, simple, cheap, -> Millipore spin filters! Cut out band, place in
filter, freeze at -70°C, thaw at 37°C, spin 10min at full speed, maybe a
wash with warm TE -> collect DNA solution; clean DNA ready to use, decent
20 min tops!
no odd chemicals, harsh conditions, No salt, glass, membranes, agarase,
low melt slime, no resin, vacuum manifolds, no electricity.etc
JUST A MICROFUGE ... A JOY TO USE!!
Dr. Baek Kim introduced me to them...
Institute of Molecular Biology
University of Oregon
More information about the Methods