methods for lysis of tissue culture cells

tbone tbone at powergrid.electriciti.com
Sun Jun 25 13:12:28 EST 1995


You could try making your lysis buffer without the SDS or urea. 
Freeze/thaw the cells to lyse them and add your DNase.  Then after the
DNase treatment, add back the SDS and urea to the appropriate final
concentration.  Best of luck.

-- 
tbone



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