DNA purification from agarose gels

Simon Baker sbaker at molbiol.ox.ac.uk
Wed Jun 28 05:17:36 EST 1995


In article <3sp9rk$1tp at lyra.csx.cam.ac.uk>, rw200 at cus.cam.ac.uk (R. Woodward) writes:
> Dear all
> 	I would like to canvas opinion on the best kit or perhaps method to use
> 	when purifying DNA fragments from agarose gels, at the moment we use the
> 	'EluQuik' kit from S&S the yield is good and so is the purity but its
> 	alittle fiddly especially if you perform alot of isolations at one time
> 	. I want to use the purified fragments in ligation reactions.
> 	Thanks to all Robert
> 
> 	R.Woodward
> 	Dept Pharmacology
> 	Tennis Court Rd
> 	University of Cambridge
> 	Email rw200 at cus.cam.ac.uk

I have just had very good results using the Promega Wizard PCR Preps kit. The 
product guide doesn't mention this as a use for these minicolumns, but it 
works well. 

Not connected with Promega in any way etc etc

Simon Baker
Dept Biochemistry
Oxford, UK



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