Shrinking Sequencing gels

WAS SHOREWA at UCBEH.SAN.UC.EDU
Thu Jun 29 07:38:31 EST 1995


Our automated DNA sequencing gels have been contracting after
polymerization and pulling away from the spacers.  We get about 300 bases
out and the buffer runs out, through the gel.  We haven't changed any
reagents or protocols in the past few months.  This problem just started
about two weeks ago.  We use BioRad acrylamide solution and
BoerhingerMannhiem  10X TBE.  

Any answers ?

Alan

uc4dna at ucbeh.san.uc.edu



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