recovery of large DNA fragments

Andrei Popov ANDREI.POPOV at bbsrc.ac.uk
Thu Jun 29 05:47:17 EST 1995


Hi
>I know that great care should be taken to avoid the shaering
> , but  23 kb
>seems a limit for the techniques I employmed. Any advise or method or reference
>for it would be
>most appreciated.

from my experience with large DNA fragments (YAC-size) I feel that
fears of shearing DNA under 50 kb are greately exagerrated. In your case
I would not bother about phenol extraction of DNA in LMA, but
 an option would be b-agarase treatment of LMA slice. 

regards

Andrei




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