pZErO-1 vector from INVITROGEN
moellere at pz350.ipsp.uni-hohenheim.de
Thu Jun 29 09:31:56 EST 1995
Anyone on the net has first hand experience with the cloning vector pZErO-1
from INVITROGEN containing the ccd-gene for positive selection? I would like
to share the experiences and I am interested to know
a. whether the vector is at least half as good as the company promises - that
means that majority (or even all?) of the transformants are really
positive recombinants with insert?
Is it possible to use another strain than the one recommended by
Invitrogen (TOP10F') for transformation without severe loss of
b. whether it is possible to makr your own pZErO-DNA by amplifying the vector
in a normal E.coli host with strong lac-repressor?
c. What is the reason for using the antibiotic zeocin? Does it have special
features or has it only to do with the fact that only Invitrogen is
selling it?The company says that it will produce less satellite colonies
than ampicillin. Any other company known which sells Zeocin?
Every comment welcome. Thanks!
Univ. of Hohenheim, Inst. Plant Breeding, Stuttgart, Germany
personal e-mail: moellere at rs1.rz.uni-hohenheim.de
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