A question on Lambda ZapII clones?

ronald goto rgoto at smtplink.coh.org
Fri Jun 30 11:09:34 EST 1995


>When I was sequencing the cDNA clones from Uni-Zap XR library, I found that

>several clones share the same small stretch of nucleotides GGCACGAG after 
>EcoRI site. Theoretically, all the DNA between EcoRI and XhoI is the cDNA 
>insert. So I am just wondering why there is this strech of nuceotides.
>If anybody happened to see this kind of things before or know what
>is going on, offer your help please.

>Thank you for your attention.

>biolc4 at jetson.uh.edu 
hello
apparently you made you cDNA via stratagene kit, if you check the Eco R1 
linker has that sequence, so your cDNA start after that sequence. 
bye
Ronald




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