A question on Lambda ZapII clones?
rgoto at smtplink.coh.org
Fri Jun 30 11:09:34 EST 1995
>When I was sequencing the cDNA clones from Uni-Zap XR library, I found that
>several clones share the same small stretch of nucleotides GGCACGAG after
>EcoRI site. Theoretically, all the DNA between EcoRI and XhoI is the cDNA
>insert. So I am just wondering why there is this strech of nuceotides.
>If anybody happened to see this kind of things before or know what
>is going on, offer your help please.
>Thank you for your attention.
>biolc4 at jetson.uh.edu
apparently you made you cDNA via stratagene kit, if you check the Eco R1
linker has that sequence, so your cDNA start after that sequence.
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