Storage of Competent Cells
R. P. Grant +44 1 865 221018
rpgrant at molbiol.ox.ac.uk
Fri Jun 30 12:01:17 EST 1995
In article <gddavis-3006950821270001 at ppp201.modems.uoknor.edu>, gddavis at essex.ecn.uoknor.edu (Greg Davis) writes:
> I have a protocol for preparing and storing E. coli cells for
> electroporation. The maxumum storage life is claimed as 6 months after
> freezing in a dry ice/ethanol bath and storing at -70oC.
> Is there a good method for long term storage of cells prepared by the
> CaCl2 method which retains high transformation efficiencies?
Our experience is that freezing in liquid nitrogen and then direct transfer to
-80 works best. Some lines can survive slight warming, but some are very
difficult (especially if your -80 goes up to -40.. :(
Richard P. Grant MA DPhil rpgrant at molbiol.ox.ac.uk
Nuffield Department of Obstetrics and Gynaecology, University of Oxford.
FAX +44 1 865 69141 TEL +44 1 865 221018
"Every four seconds somewhere in the world someone gives birth.
Gentlemen, we have to find this woman and stop her."
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