Question: What does BSA do in PCR reactions?

kang at msvax.mssm.edu kang at msvax.mssm.edu
Wed Mar 1 03:35:12 EST 1995


In article <3j093q$3g3 at hydra.acs.ttu.edu>, Z3C20 at ttacs3.ttu.edu (Yi, Xiaoming) writes:
>In <3iv5d5$nq5 at gensva.athena.livjm.ac.uk> M.Willcox at livjm.ac.uk writes:
>
>> Hello,
>> 
>> Could someone please tell me what BSA does in PCR reactions?
>> 
>> Many thanks,
>> 
>> Mark
>
>I think BSA can stabilize the enzyme(Taq), BSA is also added to restriction
>enzyme digestions to stabilize the enzymes, since adding BSA will increase the total
>concentration of protein in the mixture 


I want to add another feature of BSA.
In addition to stabilizing ptrotein by making high concentration of protein,
BSA will bind and trap the trace amount of SDS which is contaminated into the
reaction system in anyway.

SDS is a very very potent inhibitor of REs or Taq.

Good Luck.




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