Church hyb solution questions

kang at msvax.mssm.edu kang at msvax.mssm.edu
Wed Mar 1 11:34:31 EST 1995


In article <3j23v2$cbr at server.st.usm.edu>, Glen Shearer <gshearer at whale.st.usm.edu> writes:
>"Dr. Kent L. Nastiuk" <nastiuk at rockvax.rockefeller.edu> wrote:
>>
>> converrl at ucbeh.san.uc.edu wrote:
>> >
>> >      I had some questions about church hybridiation solution:
>> > 
>> > 1. What is the original reference? (I have tried like anything to locate it & I
>> > cannot)
>> 
>> Church, G.M. and Gilbert, G.  (1984)  Genomic Sequencing.  PNAS 81:1991-1995
>> 
>> hope that helps
>> 
>
>We've used the following slight modification of the hyb soln' for
>years with very good results.
>
>0.5 M sodium phosphate pH 7.0
>5% w/v SDS (use the cheap 95% pure stuff from Sigma)
>
>We usually mix this up from 1M NaPO4 stock and 20% SDS stock.
>I think the semi-pure SDS works better than the expensive SDS  ;)
>
>Glen
>[gshearer at whale.st.usm.edu]
>

Hi Glen;

Would give me the recipie of 0.5M Sodium phosphate pH 7.0?
I am a little lazy to calculate the ratio of mono and di basic/.

Thanks.

Chulho Kang.




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