Transfecting Primary Cell Lines

[Martin D. Leach]

In article <dcox.2.000C374A at medmicro.uct.ac.za>, dcox at medmicro.uct.ac.za
(Dermot Cox) wrote:

> We are at present having problems transfecting primary cells derived from 
> foetal bovine testes with plasmid DNA.  We are using the DOTAP kit from 
> Boehringer-Mannheim with little success.  Has anyone had similar
problems with 
> this kit or could make suggestions of alternate methods.
> 
Some cell types are virtually non-transfectable...one example is primary
rat cerebellar granule cells (in our hands)...

although they have been reported to be transfected (sorry sentence
construction is down...since i am abstaining from caffeine) using DOGS (a
component of transfectam-made by promega) or using a retroviral mediated
vector...

Martin

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