Transfecting Primary Cell Lines

Dermot Cox dcox at
Wed Mar 1 15:57:08 EST 1995

We are at present having problems transfecting primary cells derived from 
foetal bovine testes with plasmid DNA.  We are using the DOTAP kit from 
Boehringer-Mannheim with little success.  Has anyone had similar problems with 
this kit or could make suggestions of alternate methods.

Dermot Cox
Dept. Med. Micro
UCT Medical School
Cape Town

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