Hi I have seen several investigators use Triton X-100 (anywhere b/w 0.5 to 2.5%) to extract SDS from gels. Wash the gels at room temperature with the Triton X-100 for at least 30 minutes with at least one exchange of detergent. Then wash the Triton X-100 with your buffer before the hemolytic overlay. Hope this helps. Julio Figueroa JFigue at nomvslsumc.edu or JFigue at aol.com