SDS Removal

JFigue jfigue at aol.com
Thu Mar 2 08:37:00 EST 1995


Hi
I have seen several investigators use Triton X-100 (anywhere b/w 0.5 to
2.5%) to extract SDS from gels. Wash the gels at room temperature with the
Triton X-100 for at least 30 minutes with at least one exchange of
detergent. Then wash the Triton X-100 with your buffer before the
hemolytic overlay. Hope this helps.
Julio Figueroa
JFigue at nomvslsumc.edu or JFigue at aol.com



More information about the Methods mailing list