smearing on pulse field gel

[GEN282E at ccs1.cc.monash.edu.au]

Hi netters,

     I am working on genomic mapping of Pseudomanas by pulse field gel 
electrophoresis. One particular strain of a species I'm working with 
produces a smear when digested within agarose plugs whereas other 
strains of the same species can be cut successfully by the same rare 
cutting enzymes such as SwaI or CeuI. However, they all can be cut by 
SpeI. Does anyone have any suggestions? Any help at all will be greatly 
appreciated.



Many thanks in advance.




WF Hong