Seeking ß-Gal plasmid with nuclear targeting signal!

Sigrid Lehnert sigrid at dance.tap.csiro.au
Thu Mar 2 23:57:27 EST 1995


In article <3ii78b$tpi at rs18.hrz.th-darmstadt.de>, dh97 at pop.th-darmstadt.de
wrote:

> Hi there!
> 
> I'm looking for a plasmid with a lacZ / nuclear targeting signal
> fusion. Since I will discriminate transfected ß-Gal activity from
> lysosomal internal ß-Gal activity such a plasmid would help me in
> this approach. Can someone help me?
> 
> Thanks in advance!
> 
> Michael
>
Dear Michael,

there are a number of such constructs around but one that has been made
available to me and works well both intransfected cells and transgenic
mice is Seong-Seng Tan's nuclear targeted lacZ plasmid. Reference: Tan,
S.-S. Dev. Biol. 146, 24-37 (1991). 

Viel Glueck!  Sigrid



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