Staining nitrocellulose

Ferenc Marincs agpfxm at pnv.palm.cri.nz
Thu Mar 2 13:53:37 EST 1995


In article <Bart_Sefton-0103951511410001 at 198.202.67.144>,
Bart_Sefton at QM.Salk.Edu (Bart Sefton) wrote:

> Try 1 microliter of Pelican India ink per ml of Tris or Phosphate-buffered
> saline containing 0.2% Tween 20.  It even stains bands on blots after they
> have been blocked with BSA and ovalbumin and incubated with antibodies. 
> Wash with water to get rid of the excess stain.

You can stain your protein blot with 0.5 % Ponceau S in 1% acetic or trichloro-
acetic (TCA) acid. This staining is reversibile, destain with water.

Ferenc Marincs
AgResearch, Private Bag 11008, Palmerston North, New Zealand
Phone: +64-6-356 8019, Fax: +64-6-351 8032
E-mail: agpfxm at pnv.palm.cri.nz



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