phef at acpub.duke.edu
Thu Mar 2 13:11:20 EST 1995
In article <D.J.Glover.34.000F3D3E at bham.ac.uk>, D.J.Glover at bham.ac.uk
(David Glover) wrote:
> I am using a Molecular Dynamics Phosphorimager to
> obtain quantitative data from northern blots. I have a couple of questions
> that other users around me have been unable to answer to my satisfaction.
> My second question regards the production of a
> publication quality hardcopy of the image. I vaguely remember some discussion
> on this newsgroup about this a long time ago.If I remember rightly the best
> solution anybody offered was to expose the blot to an autorad. after exposure
> to the phosphor screen and photograph it.
- scan cassette at the highest resolution (88 microns)
- bring the image into your favorite TIFF-friendly draw program,
- print on 600 dpi laserprinter (we use HP 4M+) using glossy HP plotter paper
- (it is still better to do an autorad and scan at 300 dpi, though.
These come out even better than the "Phosporimages" using the
glossy-paper-trick. At first glance you can't tell it from a photo print.)
[I can't take credit for this, it was suggested by the Molecular
Dynamics tech rep who came to demo a Fluorimager we didn't buy]
Developmental, Cell, and Molecular Biology Group
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